The interest of mitochondrial DNA coding region SNPS

Abstract

As analysis of mtDNA hypervariable regions is a laborious technique, it is desirable to type mtSN Ps before performing sequencing. This study presents a 10 SN P multiplex, selected from mitochondrial coding region loci. These markers can subdivide any population samples in H* and non-H* haplogroups, assigning non-H* haplogroups, which in same cases is useful for forensic casework. The SN P multiplex detected 13 different non-H* haplogroups encountered in the two most common populations in our casework – European and African ancestry populations. Haplogroups U, J, T and L1/ L2 are the most frequent non-H* haplogroups in the Portuguese population samples. A probable new variant was assigned as a U/K haplogroup. This coding SN P assay is a simple method and can increase the discrimination of hypervariable region haploypes