Please use this identifier to cite or link to this item: https://hdl.handle.net/10316.2/31858
Title: Proteomic investigation of tissues of medical interest by MALDI MSI
Authors: Djidja, M. C.
Clench, M. R.
Loadman, P. M.
Sutton, C. W.
Scriven, P.
Claude, E.
Snel, M. F.
Franck, J.
Salzet, M.
Burrell, M.
Francese, S.
Issue Date: 2010
Publisher: Imprensa da Universidade de Coimbra
International Academy of Legal Medicine
Journal: http://hdl.handle.net/10316.2/2632
Abstract: MALDI Mass Spectrometry Imaging (MALDI MS I) enables images of the distribution of endogenous/xenobiotic compounds to be obtained directly from intact tissue sections. The ability to localise thousand of ions in a single analysis, without using radioactive probes or antibodies, makes it a very powerful tool in biomarker discovery. Technological improvements of in situ proteomic protocols have recently enabled MALDI MS I to be used for the identification of up/down regulated species in both snap frozen and archived tissues such as formalin fixed paraffin embedded (FFPE) samples. A further improvement of these protocols, the incorporation of a surfactant in the enzymatic digestion step, is discussed for the investigation of protein distribution and expression from frozen and FFPE adenocarcinoma tissue sections
URI: https://hdl.handle.net/10316.2/31858
ISBN: 978-989-26-0173-1 (PDF)
DOI: 10.14195/978-989-26-0173-1_57
Rights: open access
Appears in Collections:Acta medicinae legalis et socialis

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