Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316.2/31858
Title: | Proteomic investigation of tissues of medical interest by MALDI MSI | Authors: | Djidja, M. C. Clench, M. R. Loadman, P. M. Sutton, C. W. Scriven, P. Claude, E. Snel, M. F. Franck, J. Salzet, M. Burrell, M. Francese, S. |
Issue Date: | 2010 | Publisher: | Imprensa da Universidade de Coimbra International Academy of Legal Medicine |
Journal: | http://hdl.handle.net/10316.2/2632 | Abstract: | MALDI Mass Spectrometry Imaging (MALDI MS I) enables images of the distribution of endogenous/xenobiotic compounds to be obtained directly from intact tissue sections. The ability to localise thousand of ions in a single analysis, without using radioactive probes or antibodies, makes it a very powerful tool in biomarker discovery. Technological improvements of in situ proteomic protocols have recently enabled MALDI MS I to be used for the identification of up/down regulated species in both snap frozen and archived tissues such as formalin fixed paraffin embedded (FFPE) samples. A further improvement of these protocols, the incorporation of a surfactant in the enzymatic digestion step, is discussed for the investigation of protein distribution and expression from frozen and FFPE adenocarcinoma tissue sections | URI: | https://hdl.handle.net/10316.2/31858 | ISBN: | 978-989-26-0173-1 (PDF) | DOI: | 10.14195/978-989-26-0173-1_57 | Rights: | open access |
Appears in Collections: | Acta medicinae legalis et socialis |
Files in This Item:
File | Description | Size | Format | |
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55-_acta_medicinae_legalis.pdf | 1.28 MB | Adobe PDF |
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