20 SNP-Plex as a complement method in paternity testing

Abstract

This study intended to examine a set of 20 autosomal Single Nucleotide Polymorphisms (SN Ps) selected from the 52-plex developed by the SN PforID Consortium for human identification and to study its usefulness in investigation of paternity cases. We designed two 10-plexes and investigated 50 paternity cases, previously examined in this laboratory with standard ST R methodologies. There was a total agreement between exclusion and not exclusion cases with the results obtained by ST R analysis, except for one case where it was not possible to exclude the father with SN P analysis, probably due to the small number of SN Ps studied. In paternity exclusions, between one and seven incompatibilities were detected for the SN P loci studied. This study demonstrates that analysis of a small number of SN P loci, as 20 polymorphisms, can be very useful in biological kinship investigation as a complement to standard ST R methodologies, being an advantage to increase the number of loci to strengthen SN P study as a complement methodology